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DUPRE Mathieu
Thème de Recherche: temp
Autre(s) thème(s) de recherche ou rattachement(s) : - temp
mathieu.dupre
univ-montp2.fr
0467143761
Bureau: 0, Bât: 17 - Site : Faculté des sciences
Productions scientifiques :
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Investigation of Silicon-Based Nanostructure Morphology and Chemical Termination on Laser Desorption Ionization Mass Spectrometry Performance
Auteur(s): Dupre M., Enjalbal C., Cantel S., Martinez J., Megouda Nacéra, Hadjersi Toufik, Boukherroub Rabah, Coffinier Yannick
(Article) Publié:
Analytical Chemistry, vol. 84 p.10637-10644 (2012)
Ref HAL: hal-00771400_v1
DOI: 10.1021/ac3021104
Résumé: We have evaluated the laser desorption ionization mass spectrometry (LDI-MS) performance of six nanostructured silicon surfaces of different morphologies and chemical functionalizations. The substrates have been synthesized either by metal-assisted etching method or by vapor−liquid−solid (VLS) growth technique. In addition to the commercial nanostructured silicon-based surface (NALDI) target plates, serving as reference, the homemade surfaces have been evaluated in mass spectrometry experiments conducted with peptide solutions mimicking tryptic digests. LDI surfaces synthesized by metal-assisted etching method were the most efficient in terms of signal intensities and number of detected peptides. The surface providing the best LDI-MS performance was composed of two nanostructured layers. Interestingly, we also observed a significant influence of the type of organic coating (hydrocarbon vs fluorocarbon) on peptide ionization discrimination.
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