CHAMIEH Joseph
Thème de Recherche: Organisation Biomoléculaire
joseph.chamieh
umontpellier.fr
0448792179
Bureau: N3G17, Etg: 3 - Site : Pôle Chimie Balard Recherche
Domaines de Recherche: - Chimie/ou physique
- Physique/Matière Condensée/Matière Molle
- Chimie/Chimie analytique
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Productions scientifiques :
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Interactions between Oppositely Charged Polyelectrolytes by Isothermal Titration Calorimetry: Effect of Ionic Strength and Charge Density
Auteur(s): Lounis F., Chamieh J., Leclercq L., Gonzales Philippe, Geneste Amine, Prelot Benedicte, Cottet H.
(Article) Publié:
Journal Of Physical Chemistry B, vol. 121 p.2684-2694 (2017)
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Monitoring Biopolymer Degradation by Taylor Dispersion Analysis
Auteur(s): Chamieh J., Biron J. P., CIPELLETTI Luca, Cottet H.
(Article) Publié:
Biomacromolecules, vol. 16 p.3945-3951 (2015)
Ref HAL: hal-01407655_v1
DOI: 10.1021/acs.biomac.5b01260
Résumé: This work aims at demonstrating the interest ofmodern Taylor dispersion analysis (TDA), performed innarrow internal diameter capillary, for monitoring biopolymerdegradations. Hydrolytic and enzymatic degradations ofdendrigraft poly-L-lysine taken as model compounds havebeen performed and monitored by TDA at differentdegradation times. Different approaches for the dataprocessing of the taylorgrams are compared, including simpleintegration of the taylorgram, curve fitting with a finite numberof Gaussian peaks, cumulant-like method and ConstrainedRegularized Linear Inversion approach. Valuable informationon the kinetics of the enzymatic/hydrolytic degradation reactions and on the degradation process can be obtained by TDA.
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Preparation and full characterization of a micro-immunoaffinity monolithic column and its in-line coupling with capillary zone electrophoresis with Ochratoxin A as model solute.
Auteur(s): Chamieh J., Faye C., Dugas Vincent, Moreau T., Vandenabeele-Trambouze O., Demesmay Claire
Conference: 36th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (HPLC) (Budapest, HU, 2011-06-19)
Actes de conférence: journal of chromatography A, vol. 1232 p.93-100 (2012)
Ref HAL: hal-00698496_v1
PMID 22078231
DOI: 10.1016/j.chroma.2011.10.061
Résumé: A micro-immunoaffinity monolithic column (μIAC) was developed and in-line coupled with capillary zone electrophoresis in a fully automated way with Ochratoxin A as test solute. The in-line micro-immunoaffinity columns based on monolithic methacrylate polymers (EDMA-GMA) were prepared in situ at the inlet end of a PTFE coated fused silica capillary by UV initiated polymerization and subsequently grafted with antibodies. These μIACs were thoroughly characterized. The synthesis of the polymeric support was first demonstrated to be reproducible in terms of permeability, surface properties and efficiency. The antibodies immobilization was then studied by a new original hydrodynamic method (ADECA) allowing the in situ quantitative determination (at a miniaturized scale) of the total amount of immobilized antibodies. The combination of this measurement with the binding capacity of the μIAC allowed, for the first time, the in situ determination of immobilized antibody activity. A total of 260 ± 15 ng (1.6 ± 0.1 pmol) of IgG antibodies/cm in 75 μm i.d. monolithic column (i.e. 18 μgmg(-1)) was obtained with (anti-Ochratoxin A/Ochratoxin A) as antibody/antigen model. 40% of the immobilized antibodies remain active corresponding to a binding capacity of 1.2 ± 0.2 pmol antigen/cm (i.e. 600 pg/cm of our test solute OTA), a very high capacity when dealing with trace analysis and with regard to the detection limits (30 pg and 0.5 pg with UV and LIF detection, respectively). The recovery yields were quantitative with negligible non-specific adsorption and allow analysis of diluted samples (1 ngmL(-1)) for a percolated volume of 10 μL. It was also demonstrated that despite the progressive denaturation of antibodies consecutive to the elution step, the binding capacity of the μIAC remained high enough to implement at least 15 consecutive analyses with the same column and in a fully automated way.
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On-Line Hyphenation of Immuno-Preconcentration and Capillary Zone Electrophoresis: Application to the Analysis of Ochratoxin A
Auteur(s): Chamieh J., Faye C., Vandenabeele-Trambouze O., Moreau T., Dugas Vincent, Demesmay Claire
Conference: HPLC 2011 - 36th International Symposium on High-Performance Liquid Phase Separations and Related Techniques (Budapest, HU, 2011-06-19)
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