- Rapid sensing of circulating ghrelin by hypothalamic appetite-modifying neurons. doi link

Auteur(s): Schaeffer Marie, Langlet Fanny, Lafont Chrystel, MOLINO François, Hodson David J, Roux Thomas, Lamarque Laurent, Verdie P., Bourrier Emmanuel, Dehouck Bénédicte, Baneres J.-L., Martinez J., Méry Pierre-François, Marie J., Trinquet Eric, Fehrentz J.-A., Prévot Vincent, Mollard Patrice

(Article) Publié: Proceedings Of The National Academy Of Sciences Of The United States Of America, vol. 110 p.1512-7 (2013)

Ref HAL: hal-00796900_v1
PMID 23297228
DOI: 10.1073/pnas.1212137110

To maintain homeostasis, hypothalamic neurons in the arcuate nucleus must dynamically sense and integrate a multitude of peripheral signals. Blood-borne molecules must therefore be able to circumvent the tightly sealed vasculature of the blood-brain barrier to rapidly access their target neurons. However, how information encoded by circulating appetite-modifying hormones is conveyed to central hypothalamic neurons remains largely unexplored. Using in vivo multiphoton microscopy together with fluorescently labeled ligands, we demonstrate that circulating ghrelin, a versatile regulator of energy expenditure and feeding behavior, rapidly binds neurons in the vicinity of fenestrated capillaries, and that the number of labeled cell bodies varies with feeding status. Thus, by virtue of its vascular connections, the hypothalamus is able to directly sense peripheral signals, modifying energy status accordingly.