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- Quantification of 5 '-monophosphate cytosine arabinoside (Ara-CMP) in cell extracts using liquid chromatography-electrospray mass spectrometry

Auteur(s): Gouy M H, Fabre H., Blanchin M.-D., Peyrottes S., Perigaud C., Lefebvre-Tournier I.

(Article) Publié: Analytica Chimica Acta, vol. 566 p.178-184 (2006)


Résumé:

A LC/MS/ESI method is described to monitor 5 '-monophosphate cytosine arabinoside (Ara-CMP) formed after incubation of an Ara-C prodrug in cell extracts. After addition of an internal standard and off-line treatment with perchloric acid, Ara-CMP is separated from Ara-C and endogeneous matrix components on a porous graphitic carbon stationary phase using gradient elution with a water/acetonitrile mobile phase containing formic acid. The results of method performances (accuracy, linearity, limit of quantification and repeatability) show that the method is acceptable for its intended use. (c) 2006 Elsevier B.V. All rights reserved.



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