Boronic acid-based autoligation of nucleic acids: influence of the nature of the 30-end ribonucleotidic strand Auteur(s): Smietana M. (Article) Publié: Chemical Monthly / Monatshefte Für Chemie, vol. 144 p.495-500 (2013) Ref HAL: hal-00821002_v1 DOI: 10.1007/s00706-012-0881-7 Résumé: The development of synthetic systems displaying dynamic and adaptive characteristics is a formidable challenge with wide applications from biotechnology to therapeutics. Recently, we described a dynamic and programmable nucleic acid-based system relying on the formation of reversible boronate internucleosidic linkages. The DNA- or RNA-templated system comprises a 50 -ended boronic acid probe connecting a 30 -ended ribonucleosidic oligonucleotide partner. To explore the dominant factors that control the reversible linkage, we synthesized a series of 30 -end modified ribonucleotidic strands. Evidence suggests that geometric and steric factors are key features for controlling the equilibria. |