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- Boronic acid-based autoligation of nucleic acids: influence of the nature of the 30-end ribonucleotidic strand doi link

Auteur(s): Smietana M.

(Article) Publié: Chemical Monthly / Monatshefte Für Chemie, vol. 144 p.495-500 (2013)


Ref HAL: hal-00821002_v1
DOI: 10.1007/s00706-012-0881-7
Résumé:

The development of synthetic systems displaying dynamic and adaptive characteristics is a formidable challenge with wide applications from biotechnology to therapeutics. Recently, we described a dynamic and programmable nucleic acid-based system relying on the formation of reversible boronate internucleosidic linkages. The DNA- or RNA-templated system comprises a 50 -ended boronic acid probe connecting a 30 -ended ribonucleosidic oligonucleotide partner. To explore the dominant factors that control the reversible linkage, we synthesized a series of 30 -end modified ribonucleotidic strands. Evidence suggests that geometric and steric factors are key features for controlling the equilibria.