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Hervé CottetProfessor in Analytical Chemistry
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Electrokinetic separation techniques
Taylor Dispersion AnalysisStudy of biomolecule interactions (stoichiometry, affinity constants)
Characterization of proteins, biopolymers, polyelectrolytes and nanoparticles
Characterization of vaccines, antigens and mRNA LNPElectrophoretic mobility modelling
Capillary coatings for protein analysis in CE and CE-MS
Dendrigraft Poly-L-lysine
Industrial and academic programs
P158. Study of interactions between gadolinium-based contrast agents and collagen by Taylor dispersion analysis and frontal analysis continuous capillary electrophoresis. C. Somnin, J. Chamieh, L. Leclercq, C. Medina, O. Rousseaux, H. Cottet, Pharmaceutics 2024, submitted.
P157. Optical determination of the internal capillary diameter used in Taylor dispersion analysis and capillary electrophoresis. S. Roca, L. Leclercq, C. Somnin, L. Dhellemmes, J. Chamieh, H. Cottet, Electrophoresis, 2024, accepted.
P156. Preventing the impact of solute adsorption in Taylor dispersion analysis: application to protein and lipid nanoparticle analysis. S. Roca, L. Leclercq, J.-P. Biron, M. Martin, H. Cottet, J. Chromatogr. A, 2024, 1736, 465325.
P155. Getting the best out of capillary electrophoresis and capillary electrophoresis–mass spectrometry by quantifying sources of peak broadening for proteins using polyelectrolyte multilayer coated fused silica capillaries. L. Dhellemmes, L. Leclercq, A. Höchsmann, C. Neusüß, M. Martin, H. Cottet, Anal. Chem., 2024, 96(38), 15205–15212.
P154. Charge variant analysis of monoclonal antibodies by CZE-MS using a successive multiple ionic-polymer layer coating based on diethylaminoethyl-dextran. A. Höchsmann, L. Dhellemmes, L. Leclercq, H. Cottet, C. Neusüß, Electrophoresis, 2024, Early view.
P153. Taylor dispersion analysis for measurement of diffusivity and size of gadolinium-based contrast agents. C. Somnin, L. Leclercq, J. Chamieh, M. Le Menedeu, C. Medina, O. Rousseaux, R. Tripier, C. Platas Iglesias, H. Cottet, Eur. J. Pharm. Sci., 2024, 200, 106831.
P152. Dual contributions of analyte adsorption and electroosmotic inhomogeneity to separation efficiency in capillary electrophoresis of proteins. L. Dhellemmes, L. Leclercq, L. Lichtenauer, A. Höchsmann, M. Leitner, A. Ebner, M. Martin, C. Neusüß, H. Cottet, Anal. Chem., 2024, ASAP article.
P151. Investigating cationic and zwitterionic successive multiple ionic-polymer layers for protein separation by capillary electrophoresis. L. Dhellemmes, L. Leclercq, H. Frick, A. Höchsmann, N. Schaschke, C. Neusüß, H. Cottet, J. Chromatogr. A, 2024, 1720, 464802.
P150. Taylor dispersion analysis using capacitively coupled contactless conductivity detector. C. Somnin, J. Chamieh, P. Saetear, H. Cottet, Talanta, 2024, 272, 125815.
P149. Size-based characterization of dendrigraft poly(L-lysine) by free solution capillary electrophoresis using polyelectrolyte multilayer coatings. S. Roca, L. Leclercq, H. Cottet, J. Chromatogr. A, 2024, 1718, 464719.
P148. Self-emulsifying drug delivery systems (SEDDS): how organic solvent release governs the fate of their cargo. A. M. Jörgensen, R. Wibel, F. Veider, B. Hoyer, J. Chamieh, H. Cottet, A. Bernkop-Schnürch, Int. J. Pharm., 2023, 647, 123534.
P147. Fast, simple and calibration-free size characterization and quality control of extracellular vesicles using capillary Taylor dispersion analysis. S. Obeid, J. Chamieh, T. D. Mai, M. Morani, M. Reyre, Z. Krupova, P. Defrenaix, H. Cottet, M. Taverna, J. Chromatogr. A, 2023, 1705, 464189.
P146. Optimization of limit of detection in Taylor dispersion analysis: application to the size determination of vaccine antigens. C. Malburet, M. Martin, L. Leclercq, J.-F. Cotte, J. Thiébaud, J.-P. Biron, J. Chamieh, H. Cottet, Talanta Open, 2023, 7, 100209.
P145. Critical parameters for highly efficient and reproducible polyelectrolyte multilayer coatings for protein separation by capillary electrophoresis. L. Dhellemmes, L. Leclercq, A. Höchsmann, C. Neusüß, J.-P. Biron, S. Roca, H. Cottet, J. Chromatogr. A, 2023, 1695, 463912.
P144. Modifying last layer in polyelectrolyte multilayer coatings for capillary electrophoresis of proteins. S. Roca, L. Leclercq, P. Gonzalez, L. Dhellemmes, L. Boiteau, G. Rydzek, H. Cottet, J. Chromatogr. A, 2023, 1692, 463837.
P143. Molecular determinants of fibrillation in a viral amyloidogenic domain from combined biochemical and biophysical studies. J. F. Nilsson, F. Gondelaud, H. Baroudi, G. Pesce, C. Bignon, D. Ptchelkine, J. Chamieh, H. Cottet, A. V. Kajava, S. Longhi, Int. J. Mol. Sci. 2023, 24(1), 399.
P142. Taylor dispersion analysis discloses the impairment of Aβ peptide aggregation by the presence of a fluorescent tag. M. Deleanu, J.-F. Hernandez, H. Cottet, J. Chamieh, Electrophoresis, 2023, 44(7–8), 701–710.
P141. Taylor dispersion analysis to support lipid-nanoparticle formulations for mRNA vaccines. C. Malburet, L. Leclercq, J.-F. Cotte, J. Thiébaud, E. Bazin, M. Garinot, H. Cottet, Gene Ther., 2022, 30(5), 421–428.
P140. Polyelectrolyte multilayers in capillary electrophoresis. S. Roca, L. Dhellemmes, L. Leclercq, H. Cottet. ChemPlusChem, 2022, 87(4), e202200028.
P139. Determination of ultrahigh molar mass of polyelectrolytes by Taylor dispersion analysis. X. Leclercq, L. Leclercq, A. Guillard, L. Rodriguez, O. Braun, C. Favero, H. Cottet. J. Chromatogr. A, 2022, 1670, 462949.
P138. Taylor dispersion analysis and atomic force microscopy provide quantitative insight on the aggregation kinetics of Aβ(1-40)/Aβ(1-42) amyloid peptide mixtures. M. Deleanu, O. Deschaume, J.-F. Hernandez, L. Cipelletti, C. Bartic, H. Cottet, J. Chamieh, ACS Chem. Neurosci., 2022, 13(6), 786–795.
P137. Size and charge characterization of lipid nanoparticles for mRNA vaccines. C. Malburet, L. Leclercq, J.-F. Cotte, J. Thiébaud, E. Bazin, M. Garinot, H. Cottet, Anal. Chem., 2022, 94(11), 4677–4685.
P136. Separation of three strains of polio virus by capillary zone electrophoresis and study of their interaction with aluminum oxyhydroxide. C. Malburet, L. Leclercq, J.-F. Cotte, J. Thiébaud, H. Cottet, J. Chromatogr. A, 2022, 1667, 462838.
P135. Generation and characterization of air micro-bubbles in highly hydrophobic capillaries. C. Renard, L. Leclercq, H. Cottet, Electrophoresis, 2022, 43(5–6), 767–775.
P134. Chemoprevention with a tea from Hawthorn (Crataegus oxyacantha) leaves and flowers attenuates colitis in rats by reducing inflammation and oxidative stress. R. de Paula do Nascimento, A. P. da Fonseca Machado, V. Silva Lima, A. M. Tomazini Munhoz Moya, L. M. Reguengo, S. Bogusz Jr, R. Franco Leal, P. Cao-Ngọc, J.-C. Rossi, L. Leclercq, H. Cottet, C. Baú Betim Cazarin, M. R. Maróstica Jr, Food Chem. X, 2021, 12, 100139.
P133. Biodegradation of metal-based ultra-small nanoparticles: a combined approached using TDA-ICP-MS and CE-ICP-MS. L. Labied, P. Rocchi, T. Doussineau, J. Randon, O. Tillement, H. Cottet, F. Lux, A. Hagège, Anal. Chim. Acta, 2021, 1185, 339081.
P132. Unraveling the speciation of β-amyloid peptides during the aggregation process by Taylor dispersion analysis. M. Deleanu, J.-F. Hernandez, L. Cipelletti, J.-P. Biron, E. Rossi, M. Taverna, H. Cottet, J. Chamieh, Anal. Chem., 2021, 93, 6523–6533.
P131. Antigen-adjuvant interactions in vaccines by Taylor dispersion analysis: size characterization and binding parameters. C. Malburet, L. Leclercq, J. F. Cotte, J. Thiébaud, S. Marco, M. C. Nicolaï, H. Cottet, Anal. Chem., 2021, 93, 6508–6515.
P130. Screening for pancreatic lipase natural modulators by capillary electrophoresis hyphenated to spectrophotometric and conductometric dual detection. G. Al Hamoui Dit Banni, R. Nasreddine, S. Fayad, P. Cao-Ngoc, J.-C. Rossi, L. Leclercq, H. Cottet, A. Marchal, R. Nehmé, Analyst, 2021, 146, 1386–1401.
SCIEX Microscale Separations Innovation Medal Award, 2024.
Junior Member of the Institut Universitaire de France (IUF), 2011–2016.
Awardee 2016 of the French Scholar Series (Peter Wall Institute for Advanced Studies and Frenh Consulate Vancouver)
Award “Chercheur d’Avenir”, Région Languedoc-Roussillon, 2010–2013.
Award from the French Chemical Society (Analytical Chemistry Division), 2006.
Lauréat ACI jeunes chercheurs (French National Research Agency), 2004–2008.
Last update: October 25, 2024.
Taylor dispersion analysis, capillary electrophoresis, polyelectrolyte complexes, characterization of polymers, nanoparticles, dendrigraft polymers, dendrimers, analysis of bacteria; analysis of polymers, drug delivery systems. Expertise en électrophorèse capillaire. Consultance en électrophorèse capillaire. Expertise and consultancy in capillary electrophoresis and Taylor dispersion analysis.
ORCiD: 0000-0002-6876-175X