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Séminaire Chimie ED459

Endohexosaminidase catalysed synthesis of glycopeptides and proteins

Prof. Antony J. Fairbanks (Department of Chemistry, University of Canterbury, Christchurch, New Zealand)

publié le

Le Jeudi 12 Juillet 2012 à 14h
UM2, salle de cours SC-16.01

The synthetic application of endohexosaminidase enzymes (e.g. Endo A, Endo M, Endo D) promises to allow ready access to a wide variety of defined homogenous glycoproteins and glycopeptides. In particular the use of N-glycan oligosaccharides that are activated at the reducing terminus as oxazolines [1] allows their high yielding attachment to almost any amino acid, peptide or protein that contains a GlcNAc residue as an acceptor. A wide variety of oxazoline donors are readily available, either by total synthesis [2] or by isolation of the corresponding oligosaccharide from natural sources and then conversion to the oxazoline in water.[3] Finally the synthetic potential of the enzymes is particularly augmented by the production of mutant glycosynthases.[4] The development of this methodology, and its application for the synthesis of a variety of defined glycopeptides of biological interest, including vaccine candidates and anti-diabetic agents, will be discussed.

Figure 1 (cliquer pour l’agrandir)


1. Fujita, M. ; Shoda, S.-I. ; Haneda, K. ; Inazu, T. ; Takegawa, K. ; Yamamoto, K. : Biochim. Biophys. Acta 2001, 1528, 9-14.
2. Rising, T. W. D. F. ; Heidecke, C. D. ; Moir, J. W. B. ; Ling, Z. ; Fairbanks, A. J. : Chem. Eur. J. 2008, 14, 6444-6464.
3. Noguchi, N. ; Tanaka, T. ; Gyakushi, H. ; Kobayashi, A. ; Shoda, S.-I. : J. Org. Chem. 2009, 74, 2210-2212
4. Heidecke, C.D. ; Ling, Z. ; Bruce, N. C. ; Moir, J. W. B. ; Parsons, T. B. ; Fairbanks, A. J. : ChemBioChem 2008, 9, 2045-2051.

Contact local IBMM : Dr. Béatrice Roy



  • Jeudi 12 juillet 2012 14:00-15:30 -

    Endohexosaminidase catalysed synthesis of glycopeptides and proteins

    Résumé : Séminaire Chimie ED459 – Anthony J. Fairbanks

    Lieu : UM2, salle SC-16.01

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