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Séminaire Chimie IBMM

Glycan and intact protein analysis by capillary electrophoresis and mass spectrometry : from CE-MS to CE-CE-MS

Prof. Christian Neusüß (Faculty of Chemistry, Aalen University, Germany)

publié le

Le Mardi 29 mars 2016 à 13h30
UM FdS, Salle de Cours SC-20.02

The separation selectivity of capillary electrophoresis (CE) in combination with the high information content of mass spectrometry results in a powerful tool for the description of complex samples. This is especially the case for the characterization of intact proteins, glycoproteins and glycans, where minor changes, i.e. the difference of one charge (and/or small changes in size) lead to electrophoretic separation.

In this presentation an overview of CE-MS for protein and glycan analysis is given including on the one hand technical aspects like applied electrolytes, coatings, and interfaces. On the other hand several applications will be presented : CE-MS allows the detailed characterization of proteoforms of hemoglobin in the clinical context or erythropoietin in the biopharmaceutical context (“biosimilars, biobetters”). The detailed analysis of these complex (glycol)proteins and antibodies is presented and discussed. Recent results of two-dimensional capillary electrophoresis coupled to mass spectrometry will conclude the presentation.

Contact local IBMM : Prof. Hervé Cottet (équipe DSBC)


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