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Séminaire Biomatériaux IBMM

Nanostructured active biomaterials for tissue engineering applications

Dr. Nadia Benkirane-Jessel, D.R. (INSERM U977 Biomatériaux & Ingénierie Tissulaire, Faculté de Médecine de Strasbourg)

publié le , mis à jour le

Le Mercredi 19 Mai 2010 à 14h
UM2, salle TD-16.05

N.B. La conférence sera donnée en français.

In recent years, considerable effort has been devoted to the design and controlled fabrication of structured materials with functional properties. The layer by layer buildup of polyelectrolyte multilayer films (PEM films) from oppositely charged polyelectrolytes[1] offers new opportunities for the preparation of functionalized biomaterial coatings. This technique allows the preparation of supramolecular nano-architectures exhibiting specific properties in terms of control of cell activation and may also play a role in the development of local drug delivery systems. Peptides, proteins, drugs or DNA, chemically bound to polyelectrolytes or Cyclodextrins (CDs), adsorbed or embedded in PEM films, have been shown to retain their biological activities[2-12]. Recently, we have demonstrated for the first time the sequential induction of nuclear and /or cytoplasmic expression products, mediated by β-cyclodextrin embedded in a PEM film[7].

In recent times, tissue engineering has merged with stem cell technology with interest to develop new sources of transplantable material for injury or disease treatment. Eminently interesting, are bone and joint injuries disorders because of the low self-regenerating capacity of the matrix secreting cells. We present here for the first time that embedded BMP-2 and CDs-TGFβ1 in a multilayered polyelectrolyte film can drive embryonic stem cells to the cartilage or bone differentiation depending on supplementary co-factors. We selected a model system made from layer by layer poly-l-glutamic acid (PlGA) and poly-l-lysine succinylated (PlLs) films into which BMP- 2 and CDs-TGFβ1 have been embedded. Our results demonstrate clearly that we are able to induce osteogenesis in embryonic stem cells mediated by growth factors embedded in a polyelectrolyte multilayer film [8].

References

1. Decher, G. Fuzzy nanoassemblies : Toward layered polymeric multicomposites. Science 277, 1232-1237 (1997).
2. Jessel, N. et al. Bioactive coatings based on a polyelectrolyte multilayer architecture functionalized by embedded proteins. Adv. Mater. 15, 692-695 (2003).
3. Jessel, N. et al. Build-up of polypeptide multilayer coatings with anti-inflammatory properties based on the embedding of piroxicam-cyclodextrin complexes. Adv. Funct. Mater. 14, 174-182 (2004).
4. Jessel, N. et al. Pyridylamino-β-cyclodextrin as a molecular chaperone for lipopolysaccharide embedded in a multilayered polyelectrolyte architecture. Adv. Funct. Mater. 14, 963-969 (2004).
5. Jessel, N. et al. Control of monocyte morphology on and response to model surfaces for implants equipped with anti-inflammatory agents. Adv. Mater. 16, 1507-1511 (2004).
6. Jessel, N. et al. Short-time tuning of the biological activity of functionalized polyelectrolyte. Adv. Funct. Mater. 15, 648-654 (2005).
7. Jessel, N. et al. Multiple and time scheduled in situ DNA delivery mediated by β-cyclodextrin embedded in a polyelectrolyte multilayer. Proc. Natl. Acad. Sci. USA 103, 8618-8621 (2006).
8. Dierich, A et al. Bone formation mediated by growth factors embedded in a polyelectrolyte multilayer film. Adv. Mater. 19, 693-697 (2007).
9. Zhang, X. et al. Transfection Ability and Intracellular DNA Pathway of Nanostructured Gene-Delivery Systems. Nanoletters. 13(8), 2432-2436 (2008).
10. Grossin, L et al. Step-by-Step Build-Up of Biologically Active Cell-Containing Stratified Films Aimed at Tissue Engineering. Adv. Mater. 20, 1-6 (2009).
11. Zhang, X. et al. Poly(L-lysine) nanostructured particles for gene delivery and hormone stimulation. Biomaterials 317, 1699-1706 (2010).
12. Facca, S. et al. Active nanostructured capsules as a new strategy for in vivo bone formation. Proc. Natl. Acad. Sci. USA 107, 3406-3411 (2010).

.

Dr. Nadia Benkirane-Jessel is the Leader of the « Active Biomaterials and Tissue Engineering » team at INSERM UMR 977, Strasbourg. INSERM U977 has 48 people and possesses an important knowledge in chemistry and physico-chemistry of macromolecules. It is also one of the world leader groups in the polyelectrolyte multilayer research domain. This team is since about seven years largely involved in the modification of biomaterial surfaces by means of « bio » functionalized polyelectrolyte films. This team has been granted ERT Status (technological research team) on 2006 and now included 8 researchers/clinicians 3 technicians and Engineers and 7 post-doctoral, doctoral, and master’s.

Dr. Nadia Jessel received her PhD from University Louis Pasteur (ULP), Strasbourg, France in 1994 for the work on Development of pseudopeptides as synthetic vaccines. Dr. Jessel (Benkirane) then held a postdoctoral position in collaboration with the Institut Pasteur, Paris, France, working on Immunotherapy HIV, and another postdoctoral position on the application of modified peptides as vaccines against FMDV in collaboration with Green port, NY. She joined the Unité INSERM U 595, now U977 in 2002 as associate Professor, and received the habilitation diploma (HDR) in 2004. Dr. Jessel got the permanent position in the INSERM 977 laboratory in 2004 and now Research Director. She possesses expertise in diverse fields of molecular and cellular biology, immunochemistry, tissue engineering and biomedical engineering. In the last 7 years, she focused her research on the bio-functionalization of multilayered polyelectrolyte architectures with emphasis on the use of these architectures to induce specific cellular responses and gain control over cell proliferation and differentiation.

Dr. Jessel is a co-author of 50 peer-reviewed publications in high impact factor journals (Proc. Nat. Acad. Sci. USA ; Nanoletters ; Biomaterials ; Adv. Mater. ; Adv. Funct. Mater ; Small…), chapters reviews and 3 international patents and more than 100 communications and conferences, she is a regular referee for a number of scientific journals (Adv. Mater. ; Adv. Funct. Mater ; Small, Biomaterials, Macromolecules etc.). The field of polyelectrolyte multilayers is one of the most studied now in material science. Our group have published more than one hundred articles in high-ranking journals in the domain of polyelectrolyte multilayers and with sum of the times cited exceeding 3000 times.

Contacts IBMM : Dr. Fabien Granier (Colcom) – Dr. Laurent Boiteau (équipe DSBC).

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