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Séminaire Chimie ED459

Recent trends in supramolecular interfacial architectures for optical biosensing

Prof. Wolfgang Knoll (AIT Austrian Institute of Technology, Vienna, Austria)

publié le

Le Jeudi 14 décembre 2017 à 14h
ENSCM, Amphithéâtre Godechot (campus Balard, 240 av. Émile-Jeanbrau)

In classical surface plasmon optical biosensors, the evanescent field of a resonantly excited surface plasmon mode probes some 150–200 nm of the analyte solution adjacent to the transducer surface. In order to maximize the sensitivity of the device for bio-affinity studies, e.g. monitoring antibody binding for the detection of specific antigens, or surface hybridization reactions, we are investigating the use of interfacial polymer architectures with area densities that considerably exceed planar arrangements through the use of three-dimensional microstructures. For biosensors based on surface plasmon fluorescence spectroscopy, this type of matrix offers the additional advantage of preventing fluorescence quenching of the chromophore labeled analyte, which is caused by the proximity of the chromophore to the acceptor states of the noble metal sensor substrate. With the introduction of long range surface plasmon modes as the exciting optical waves which extend much farther into the analyte solution (<1μm), substantially thicker sensor matrix layers are required. We present results that demonstrate that functionalized hydrogels are very well suited to meet the demands of these novel bio-sensor platforms.

It is further found that these hydrogels in addition to being excellent matrices for the covalent attachment of ligands or antigens for affinity studies can also guide optical modes which then probes either the effective refractive index change of the guiding matrix upon binding of the analyte molecules or can be used for the excitation of chromophore-labeled analyte molecules in ultra-sensitive fluorescence detection.


1. C.J. Huang, J. Dostalek, W. Knoll, Long range surface plasmon and hydrogel optical waveguide field-enhanced fluorescence biosensor with 3D hydrogel binding matrix : on the role of diffusion mass transfer. Biosens. Bioelectronics 2010, 26, 1425–1431.
2. C.J. Huang, J. Dostalek, A. Sessitsch, W. Knoll, Long-range surface plasmon-enhanced fluorescence spectroscopy biosensor for ultrasensitive detection of E. coli O157:H7. Anal. Chem. 2011, 83, 674–677.
3. K.Sergelen, S. Fossati, A. Turupcu, C. Oostenbrink, B. Liedberg, W. Knoll, J. Dostalek, Plasmon field-enhanced fluorescence energy transfer for hairpin aptamer assay readout. ACS Sensors 2017, 2, 916–923.

Contact local ICGM : Prof Joël Chopineau (équipe MACS)


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